Circular dkhroism measurements show that C·C+ base pairs can coexist with A·T base pairs between antiparallel strands of an oligodeoxynudeotide double-helix
Identifieur interne : 004E50 ( Main/Exploration ); précédent : 004E49; suivant : 004E51Circular dkhroism measurements show that C·C+ base pairs can coexist with A·T base pairs between antiparallel strands of an oligodeoxynudeotide double-helix
Auteurs : Donald M. Gray [États-Unis] ; Tao Cui [États-Unis] ; Robert L. Ratliff [États-Unis]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1984.
Abstract
We have studied the coil-to-helix transition of the DNA oligomer d(C4A4T4C4), using circular dichroism measurements to monitor the formation of A.T base pairs within the central self-complementary A4T4 region and the formation of protonated C·C+ base pairs at the ends of the oligomer. We found that both A·T and C·C+ base pairs formed in a coordinated fashion as the temperature and pH were lowered. The CD data of the helix form of the oligomer were consistent with the presence of paired oligomers, but not with hairpin loops. The pKa for formation of C·C+ base pairs between the C4 ends of the oligomer was higher than the pKa for formation of C·C+ base pairs in d(C8), indicating that the formation of C·C+ base pairs in the oligomer was Influenced by the presence of a paired A4T4 region. We conclude that A·T and C·C+ base pairs coexist in the self-complex of the oligomer and, therefore, that C·C+ base pairs can form between antiparallel DNA strands.
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DOI: 10.1093/nar/12.19.7565
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Gray</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Program in Molecular Biology, University of Texas at Dallas Box 830688, Richardson</wicri:cityArea></affiliation></author><author><name sortKey="Cui, Tao" sort="Cui, Tao" uniqKey="Cui T" first="Tao" last="Cui">Tao Cui</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Program in Molecular Biology, University of Texas at Dallas Box 830688, Richardson</wicri:cityArea></affiliation></author><author><name sortKey="Ratliff, Robert L" sort="Ratliff, Robert L" uniqKey="Ratliff R" first="Robert L." last="Ratliff">Robert L. Ratliff</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Genetics Group, Life Sciences Division, Los Alamos National Laboratory Box 1663, Los Alamos, NM 78545</wicri:regionArea><wicri:noRegion>NM 78545</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nucleic Acids Research</title><idno type="eISSN">1362-4962</idno><idno type="ISSN">0305-1048</idno><imprint><publisher>Oxford University Press</publisher><date type="published">1984</date><biblScope unit="vol">12</biblScope><biblScope unit="issue">19</biblScope><biblScope unit="page" from="7565">7565</biblScope><biblScope unit="page" to="7580">7580</biblScope></imprint><idno type="ISSN">0305-1048</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">We have studied the coil-to-helix transition of the DNA oligomer d(C4A4T4C4), using circular dichroism measurements to monitor the formation of A.T base pairs within the central self-complementary A4T4 region and the formation of protonated C·C+ base pairs at the ends of the oligomer. We found that both A·T and C·C+ base pairs formed in a coordinated fashion as the temperature and pH were lowered. The CD data of the helix form of the oligomer were consistent with the presence of paired oligomers, but not with hairpin loops. The pKa for formation of C·C+ base pairs between the C4 ends of the oligomer was higher than the pKa for formation of C·C+ base pairs in d(C8), indicating that the formation of C·C+ base pairs in the oligomer was Influenced by the presence of a paired A4T4 region. We conclude that A·T and C·C+ base pairs coexist in the self-complex of the oligomer and, therefore, that C·C+ base pairs can form between antiparallel DNA strands.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Gray, Donald M" sort="Gray, Donald M" uniqKey="Gray D" first="Donald M." last="Gray">Donald M. Gray</name></region><name sortKey="Cui, Tao" sort="Cui, Tao" uniqKey="Cui T" first="Tao" last="Cui">Tao Cui</name><name sortKey="Ratliff, Robert L" sort="Ratliff, Robert L" uniqKey="Ratliff R" first="Robert L." last="Ratliff">Robert L. Ratliff</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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